When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andThe Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Plate reader instructions Computer. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. A control well was set up to verify the accuracy of the standard curve. Align the two slots on the bottle holder with the two pegs on the rail. EMax. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. After reading the ELISA plate on both readers, each standard curve was nearly identical. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. All stand-alone instrument functions can be controlled using the software. Microplates for applications that require high sample recovery rates. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. 100 fmol 1536-well (8 µL) 0. and the A1 corner of the plate adapter (Figure 1-2). 96- and 384-well microplates. x. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 384-well TC plate, Poly-d-Lysine, Flat Clear. For biochemical assays, the washer removes unbound material and unreacted reagents. +1 800. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. EMax. 0 . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The same measurement settings can be used for subsequent plates as long as the volumes, tracer and concentrations remain the same. Plate readers avoiding cross-talk are recommended for luminescence measurement in 384 well plates. $245. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. #S1207. 3. x. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Following the flushing of the machine as described in Section 3. Temperature control: 4°C above ambient to 45°C. smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader, spectramax i3x. Related products. assay volume in a 384-well plate 0200-7017POS Alpha 384 STD 96 and 384-well plates < 100 amol (384-well) 1 0200-7018POS Alpha 384 HTS 96 and 384-well plates < 100 amol (384-well) 1 0200-7019POS Alpha 1536 HTS 96, 384, and 1536-well plates < 100 amol (384-well) 1 HTRF Cisbio HTRF detection with optimized Xenon light source and 616, 665 nm EM filters The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. 039. using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Bioz Stars score: 86/100, based on 1 PubMed. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. ‘StimB’ is Stimulation Buffer, included in the IP-One Gq kit. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. Products : Ionworks HT, Ionworks Quattro. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. the use of a reader calibrated with primary NIST and NMI standards. With optimized reagents, option to operate the. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. X. Step 1: Open the SoftMax Pro IMAP FP protocol. flat meniscus), but is approx 15 mL. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Analyst, M5, Flex 3, and SpectraMax L readers work differently. were pipetted into a 96-well black-walled μclear plate. Our microplate. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. We would like to show you a description here but the site won’t allow us. For more sample throughput, both readers can be easily. x. For more sample throughput, the system canSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. #N207) LAL Reagent Water (Lonza cat. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. RUO. Efficiency. VMax . Therefore, it is recommended that the pipettor height to be set to correspond to the fluid volume in the cell plate prior to compound addition. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. 0. 020 fmol 0. Find our technical support information along with contact details. In general microplate washers are designed to leave only 2 microliters of residue per well and operate with an accuracy of 3% CV per well and a uniformity of 4% CV across a plate. Read one sample or up to 384. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Validated by Molecular Devices for the FLIPR reader. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). Experience the identifiably different. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. These lab workhorses are used in drug discovery, research, bioassay validation, and. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. SpectraMax® M2 and M2e Microplate Readers are the standard for UV/visible multimode reader absorbance, providing ultrafast and full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (T onset, T m, T agg) and colloidal stability (A 2 /B 22,. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. 384-Well Microarray/High Sample Recovery Plate. 20 attomol ATP per well. Dimensions 128 x 86 x 15 mm. Figure 1. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. There is no product in the shopping cart, buy it!. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. 5 ng per well. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. x. Reliable performance with a proven track record. x. For fluorescence intensity assays, the SpectraMax® M2 and M2e units feature an. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. 96-, 384-, and 1536-well microplates. Dimensions: 18. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. 3860 N First Street. 96- and 384-well microplates. x. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. x. Turn the. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. 2. Application Area: Spectrophotometer for molecular biology. Shaking: Linear and Orbital. Analyst, M5, Flex 3, and SpectraMax L readers work differently. 4 decades . Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. #6590-0144; includes Enhanced TRF Module, excitation and emission filters). The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. 25mm: Plate height:Absorbance was measured at 490 nm with SpectraMax Plus 384 plate reader (Molecular Devices, San Jose, CA). Instrument pricing. Instructions for adding new plates: CSI/CP2: Add plate. The semi-automated PX1 PCR Plate Sealer delivers reliable uniform sealing of a range. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Microplate readers are commonly used in life science laboratories. (blue) and DsRed (red) transfected cells in 384-well plates, read from the bottom. Alpha 384 HTS 96- and 384-well . …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. 1. Molecular Devices has provided scientists with tools to expand the boundaries of their research. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. All other trademarks herein are the property of Molecular Devices, Inc. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. e. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 20 attomol ATP per well. Outer wells aligned for proper viewing with FLIPR Ideal. All stand-alone instrument functions can be controlled using the software. 01% BSA starting at a concentration of 1 x 10 6 fg/well was prepared, and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. 0200-6262. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. 5 mP 1536–2. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. Determine concentration using the Beer-Lambert LawMolecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life. NanoQuant plate. Fluorescence Microplate Reader Comparison Chart. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). M5. B. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. Application Area: Multi mode plate reader. • Tyrosine Kinase Plate Please contact your Molecular Devices sales representative. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. 5–25 µl. M2. Microplate readers can be used to quantify this turbidity to monitor events such as cell growth or to perform solubility screens. Microplate format. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. The VICTOR Nivo. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. The 384-well plate is limited to a maximum volume of a 30 µl addition in a much smaller diameter well, and using the 96. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 더 많은. Add the new plate. 315 can't find zero order. The SpectraMax 190 reader can be integrated with Molecular . It also integrates with the. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Note: These settings are based on necessary cookies. Next-generation luminescence plate reader. SpectraMax iD5. In addition, SoftMax Pro SoftwareThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. A = 2 - log 10 (%T). #3-000-510)Corning® 384 well microplate, low volume poly-D-lysine coated surface, black polystyrene, round bottom, sterile, lid, pack of 10; Synonyms: 384 multiwell plates,384 well low volume plates,384 well microplates,384 well microtiter plates,384 well plates,384 well tissue culture treated plates; find -CLS3643 MSDS, related peer-reviewed papers,. It is more inert and reduces compound interference. 4 decades . Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. #3-000-510)Page 154: Ordering Parts And Accessories. Danaher Inc 384 well plate 384 Well Plate, supplied by Danaher Inc, used in various techniques. 384-well plates • Automated rinse reduces clogging • Quiet and efficient. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. The user-friendly MultiWash™+ Microplate Washer from Molecular Devices, one of the pioneers in microplate detection, includes 20 preset protocols, works with both 96- and 384-well plates, and comes with 4 different wash/rinse bottles for out-of-the-box use. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. (or plate reader). Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 96- and 384-well. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 96/384-well plate formats. 057, depending on lot number. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). Molecular Devices: n/a:. Overview of 384 Well SBS. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Comprehensive solution. Another consideration, says Amouretti, is plate format. x. Biotek Synergy 2 Plate Reader Multi-Mode. Important things to consider when purchasing a multimode microplate reader. Extended linear dynamic range. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. Round well design with a solid black flat bottom2. QC tools and IQOQ services. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices,. 340 PC384. Molecular Devices products are warranted to meet the stated specifications. 96-, 384-, and 1536. UV absorbance detection. Ensure data accuracy of your reader. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. x. 0 . Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. Packed 25 plates per sleeve, 2 sleeves per case. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0 out of 5 $ 8,495. 58% for the detection module and 2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. plates < 100 amol (384-well) 1 0200-7018. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Microplate is validated by molecular devices for the FLIPR reader. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. Devices PathCheck® Sensor—the only patented technologySpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices). Uncontrolled Copy for Training and Reference Purposes Only . Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. DynaPro ™ Plate Reader III. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. ©2022. A. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. use as little as 2 µL samples with 24- or 64-well plates. Dynamic range > 6 decades . Figure 1. This kit is designed for customers who. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Efficient washing is achieved with cross-wise aspiration, reducing residual volume . A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. For SpectraMax L or LMax II, LMax II 384 - no adapter used. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Streamline GLP/GMP compliance. It. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. All stand-alone instrument functions can be controlled using the software. Find regional support. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. x. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. com Check our website for a current listing of worldwide distributors. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. for ELISAs and most cell-based assays. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. flat meniscus), but is approx 15 mL. Supplier: Molecular Devices. We would like to show you a description here but the site won’t allow us. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. For more sample throughput, the system canThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Description. Select Consumables. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Page 1 of 8 . The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. To prevent evaporation, the microplate was covered with a qPCR seal. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. If the computer is connected to the Internet: Select Search Automatically for. Compact design. Published Dec 16, 2020 | Updated Dec 16, 2020. Automated live cell imaging – cell counting and confluence. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. Plus 384. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Formats available are strip washers, full plate washers and combination washer-dispensers. • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. . 635. 96- and 384-well . Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. SpectraMax i3x. Get Quote . Temperature control: 4°C above ambient to 45°C. This can increase throughput and efficiency, thus saving time and space in the lab. Add 10 µL of ADP Detection Mix to those rows. • The FlexStation™ II instrument is designed specifically for 96-well microplates. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. To ensure accurate cell numbers for the cell standard curves, cells were assayed immediately. 2. A control well was set up to verify. These advertising cookies will no longer be set for this browser and device. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Temperature Control: Ambient +5C up to 42C. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. External Dimensions without lid (mm). Microplate Spectrophotometer. The washer has a small footprint and is vacuum and pressure free, The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Plus 384. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. 96/384-well plate formats. #PLUS 384) Methods. efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6 to 384 wells, the SpectraMax iD3 reader is the complete solution. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. For a higher . 5in (46. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. Two high-energy xenon flash lamps: Reading capabilities. Both readers were heated to 37ºC prior to setting up the assay. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. The color touch screen provides a visual user interface, making programming fast and intuitive. 00. FlexIII. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Selects either 96-well or 384-well plate for a stand-alone read. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. 9. Pfa1 and Pfa1 Gpx4-KO cells stably overexpressing murine FSP1 were seeded on separate 384-well plates (500 cells per well), and screened with a library of small-molecule inhibitor compounds, as. 1. 5 fg per well to 0. Tb. #4TI-0655) Microplates. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. x. 00. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. † Bottom mounting for SpectraMax Paradigm Readers only. Description. , April 14, 2022 – Molecular Devices, LLC. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum amount of DNA needed. REF - For the SpectraMax Plus 384, press to do a Cuvette Reference correction method read of buffer, water, or air taken in the cuvette that is used as I to calculate Absorbance or % Transmittance with the PathCheck Pathlength Measurement Technology. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Packed 25 plates per sleeve, 2 sleeves per case. * Note: Programming the washer with a final aspirate height of 4. Outer wells aligned for proper viewing with FLIPR. Outer wells are aligned for proper viewing with FLIPR. Gemini EM Reader scans.